Journal: Theranostics

Article Title: Ganglioside enhances the immunogenicity of nanoparticles displaying short synthetic tumor neoepitopes and epitopes

doi: 10.7150/thno.128187

Figure Lengend Snippet: CPQ/GM3 liposomal E7 peptide vaccine retarded the TC-1 tumor growth and prolonged the survival of mice. Mice were inoculated with 1x10 TC-1 tumor cells subcutaneously on day 0 and immunized with the indicated E7 vaccine, 2 µg per mouse per injection intramuscularly on day 8 and day 15. A) Average tumor volume. B) Tumor volume on day 36. C) Percentage of mice with tumor diameter under 10mm. Arrows indicate vaccination time points. Error bars show mean ± std. dev. for n = 4 per group. Figure A is analyzed by mixed-effects analysis followed by Tukey's multiple comparisons test. *, **, ***, and **** indicate P ≤ 0.05, 0.01, 0.001, and 0.0001, respectively. Figure B was analyzed by one-way ANOVA with Tukey's multiple comparisons test. Figure C was analyzed by Log-rank (Mantel-Cox) test.

Article Snippet: Renca tumor cells and TC-1 tumor cells were purchased from the ATCC and cultured in Dulbecco's modified Eagle's medium and RPMI medium separately with 10% fetal bovine serum (FBS) and 1% 100 U/mL penicillin and 100 μg/mL streptomycin.

Techniques: Injection